XB-IMG-170646
Xenbase Image ID: 170646
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Fig. 3. Xenopus laevis RARβ2 promoter elements are required for RA responsiveness. Luciferase reporters were selectively mutated for the canonical (C)
direct repeat 5 (DR5) (Sucov et al., 1990), upstream DR5 and upstream half-site (HS). Embryos were injected unilaterally at the 2- or 4-cell stage with 50 pg
reporter DNA then treated at blastula stage with 0.1 μM TTNPB or vehicle (0.1% ethanol). Embryos were collected at neurula stage (each data point represents
one pool of 10 embryos). Data are represented either as relative light units measured by the luminometer or fold induction relative to vehicle using standard
propagation of error (Bevington and Robinson, 2003). TTNPB responsiveness is reduced by mutating either the canonical DR5 or upstream DR5. Both basal
reporter activity and TTNPB responsiveness is reduced by mutating the upstream half-site; however, fold induction is equivalent to wild type. Error bars indicate
s.e.m. An unpaired t-test in GraphPad Prism v5.0 is reported (***P≤0.001; **P≤0.01). Image published in: Janesick A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |