XB-IMG-170647
Xenbase Image ID: 170647
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Fig. 4. Somite morphology and migration of hypaxial
muscle migration are disrupted in Rarβ2 MO-injected
tadpoles. (A-C) Embryos were microinjected bilaterally at the
2-cell stage with 26 ng Rarβ2.L+26 ng Rarβ2.S MOs or 52 ng
control MO. (A,B) Rarβ2 MOs result in paralysis and curved body
axis. Myod marks the somites that are thicker and fewer in
number without v-shape morphology (17/18 embryos)
compared with control MO. Red arrowheads indicate migrating
hypaxial myoblasts in controls, not observed in Rarβ2 MOembryos.
(C) Higher magnification of blurred/disorganized
somite morphologies observed in some embryos marked by
Myod in control and Rarβ2 MO-injected embryos.
(D-G) Embryos were microinjected unilaterally at the 2- or 4-cell
stage with 26 ng Rarβ2.L+26 ng Rarβ2.S MOs. (D,E) The
injected side displays thicker disorganized somites (marked by
Myod) without v-shape morphology (20/21 embryos), compared
with the uninjected side. Red outline indicates melanophores
and migrating hypaxial muscle that are absent on the injected
side. (F,G) The injected side shows diminished hypaxial Tbx3
expression (11/12 embryos), compared with the robust hypaxial
migration on the uninjected side (red arrowheads). All embryos
are shown in lateral view at stage 40; anterior on the left. Image published in: Janesick A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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