XB-IMG-170648
Xenbase Image ID: 170648
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Fig. 5. Somite number is reduced and length increased in
Rarβ2 MO-injected embryos. (A-F) Embryos were
microinjected unilaterally at the 2- or 4-cell stage with 26 ng
Rarβ2.L+26 ng Rarβ2.S MOs or 52 ng control MO. (A,B) Two
lateral sides of the same embryo are shown at stage 26; anterior
on the left. Injected side is indicated by magenta β-gal lineage
tracer. Rarβ2 MOs (B) disrupt and disorganize the chevronshaped
somite morphology, reduce somite number and increase
somite thickness (18/18 embryos) compared with the uninjected
side (A), as indicated by Myod expression. (C-F) Higher
magnification of somite morphologies (marked by Myod) on the
Rarβ2 MO-injected side observed in different embryos.
(G,H) Paraffin wax-embedded coronal sections of embryos from
(A-F). (I) Somite number is quantitated from sectioned embryos;
each data point represents one embryo (n=7). (J) Somite size
(length from posterior to anterior end) is quantitated from
sectioned embryos using ImageJ (units are distance in pixels);
each data point represents one somite. R, rostral somites; C,
caudal somites. Statistics for I,J were calculated in GraphPad
Prism v5 using a t-test (*P≤0.05; ***P≤0.001). Image published in: Janesick A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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