XB-IMG-170650
Xenbase Image ID: 170650
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Fig. 7. Tbx6 and Tbx3 are differentially regulated by
Ripply2 in vivo. Whole-embryo luciferase assay reflecting
Tbx6 or Tbx3 transcriptional activity in the presence or
absence of wild-type or mutant Ripply2 (Bowline or
Ledgerline). Each data point represents one pool of five
embryos, collected from different clutches of females (as
indicated). Error bars indicate s.e.m. One-way ANOVA and
Bonferroni’s multiple comparison test was conducted using
GraphPad Prism: ###P≤0.001 relative to reporter alone;
***P≤0.001 and *P≤0.05 relative to reporter+Tbx6 mRNA.
Tbx6 increases activity ∼3-fold. Ripply2 (Bowline or
Ledgerline) mRNAs repress activity to basal levels when coinjected
with Tbx6 mRNA; microinjection of Ripply2 (Bowline
or Ledgerline) mRNA mutated (Mut) for the WRPW and
FPVQ domain does not repress Tbx6 reporter activity. Tbx3
reduces activity by about 90%, while Ripply2 (Bowline or
Ledgerline or mutants) does not affect Tbx3 reporter activity. Image published in: Janesick A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |