Xenbase Image ID: 170720

Figure 2. Pcdh15 morphant larvae have altered mechanoelectrical transduction channels and photosensory deficits. (A) Uptake of FM1–43 dye by the hair cells of cranial and caudal neuromasts (high magnification of a neuromast, with 6–12 sensory hair cells shown). Dye uptake in neuromasts (some are outlined) is much weaker in 3-dpf pcdh15 morphants than it is in controls: 120.6 ± 15.5 µm2 in morphants (mean ± SEM; n = 33), versus 471.2 ± 37.5 µm2 in controls (n = 21; unpaired t test, ***, P < 0.0001). (B, top left) Representative electroretinogram traces (flash intensity increasing from bottom to top). (bottom left) The time-to-peak values obtained for the a- and b-waves (implicit times) did not differ significantly between controls and morphants. (right) Photoresponse curves (V, μV), plotted as a function of flash intensity and fitted with the Naka–Rushton function, in morphants and controls. The responses show a significant attenuation in morphants (comparison of fits by the least-squares method: P < 0.0001 for both waves), whereas after normalization by the maximum value Vmax, they do not differ significantly, giving similar values of I0.5 (P > 0.5 for both waves; bottom right panel; n = 7 controls, 13 morphants). Bars, 20 µm. Image published in: Schietroma C et al. (2017) © 2017 Schietroma et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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