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Figure 3. Effects of Ba2+ on ionic currents in oocytes expressing different AQPs. (A) Superimposed current traces as a function of time measured by voltage clamp at maximal activation in divalent-free saline (upper row) and after perfusion with bath saline containing 1 mM BaCl2 (lower). (B) Current-voltage relationships in Ba2+ for traces presented in (A). (C) Box plot summary of compiled data for AQP1, PIP2;1, PIP2;2 and BIB channels before and after 1 mM Ba2+ application. (D) Histogram showing the relative outward rectification values (I+60/I−80) as mean ± SEM. Ratios were calculated as amplitude of outward (+60 mV) to inward (−80 mV) currents. **** (p < 0.0001); ** (p < 0.001); * (p < 0.01); NS (not significant); using ANOVA with post-hoc Bonferroni tests. n values are near the x-axis.

Image published in: Kourghi M et al. (2017)

© 2017 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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