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Figure 5. Effects of sequential application of Ca2+ followed by Ba2+ on ionic current responses in oocytes expressing different classes of AQPs. (A) Superimposed current traces under voltage clamp showing the maximal responses of AtPIP2;2, AtPIP2;1, HsAQP1 and DmBIB expressing oocytes recorded in divalent-free saline (left), after perfusion of saline containing the indicated amount of free Ca2+ (middle), and after application of Ba2+ in the continuing presence of the same concentration of Ca2+ (right). (B) Current-voltage relationships for traces shown in (A). (C) Summary box plot of conductance values for AtPIP2;2, AtPIP2;1, HsAQP1 and DmBIB expressing oocytes in different divalent cation salines. *** (p < 0.001); ** (p < 0.01); * (p < 0.05); NS (not significant); using ANOVA with post hoc Bonferroni tests; n values are below the x-axis. Image published in: Kourghi M et al. (2017) © 2017 by the authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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