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Figure 2—figure supplement 4. Complementation of brush by overexpressing AtCNGC19 or AtCNGC20.(A) Nodulation and rhizobial infection assay upon hairy root transformation of brush with T-DNA constructs leading to overexpression of AtCNGC19 and AtCNGC20 or with an empty vector as a control. (B) Box plot showing the number of DsRED-positive nodules per transformed plant (n = 10 for all constructs). (C) Yeast two-hybrid interaction assay with the BRUSH N-terminus (BRUSH NT) fused to the GAL4 binding domain (BD) and either AtCNGC19 NT or AtCNGC20 NT fused to the GAL4 activation domain (AD). Yeast cells were resuspended in water (OD600= 0.5 and 0.05) and spotted onto -LW (leucine, tryptophan), -LWH (leucine, tryptophan, histidine) and -LWAH (leucine, tryptophan, adenine, histidine) solid media. The lower panel in (A) contains images of the DsRED channel to visualize Mesorhizobium loti. Roots were observed 6 weeks after the addition of rhizobia. Scale bars in (A) represent 2 mm. Different letters in (B) indicate different statistical groups (ANOVA followed by Tukey’s HSD test, F(2, 27)=20.94, p < 0.001). Image published in: Chiasson DM et al. (2017) © 2017, Chiasson et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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