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Figure 2—figure supplement 5. CNGC.IVA gene expression in Lotus japonicus roots after rhizobial inoculation.(A), Expression of CNGC.IVA cluster genes in roots as analyzed by RT-qPCR on Day 0 (●) and Day 12 (○) after inoculation with Mesorhizobium loti. Each point represents a biological replicate. Shown is the relative expression as calculated with the 2△△CT method using eEF-1Aα as a reference. (B) Promoter activity of CNGC.IVA genes three weeks after inoculation with Mesorhizobium loti. The 2 kb promoter sequence of each gene was fused to GUS and introduced into Gifu wild-type roots by hairy root transformation. Expression of GUS was detected in developing nodules (arrows) after overnight staining. Scale bars in (B) represent 1 mm. Image published in: Chiasson DM et al. (2017) © 2017, Chiasson et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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