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Figure 2—figure supplement 6. BRUSH expression in roots during nodulation.(A) BRUSH 2 kb promoter activity was not evident in the root susceptible zone prior to inoculation. After inoculation with Mesorhizobium loti, promoter activity was observed in infected root hairs (B, arrow), nodule primordia (C, arrow) and infected nodule cells (D, arrow). The promoter sequence was fused to GUS and introduced into Gifu wild-type plants by hairy root transformation. Roots were harvested 3 weeks after inoculation with rhizobia, stained for GUS activity overnight, and counterstained with ruthenium red after sectioning (B,C,D). Scale bars represent 0.6 mm (A), and 0.2 mm (B,C,D). Image published in: Chiasson DM et al. (2017) © 2017, Chiasson et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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