XB-IMG-170751
Xenbase Image ID: 170751
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Figure 3—figure supplement 2. Brush interaction in oocytes and permeability to Ca2+ and K+.(A) Bimolecular fluorescence complementation (BiFC) analysis of BRUSH and brush fused to either a N-terminal (VN) or C-terminal (VC) fragment of Venus (YFP). Shown are representative oocytes 3 days after co-injection of the corresponding cRNAs. The bottom right image of each panel (VN fusion alone) displays the (non-detectable) background oocyte fluorescence. (B) Control BiFC experiments utilizing AtKAT1. Images are overlays of bright-field and fluorescent images. (C) Current responses of an oocyte expressing BRUSH-YFP in the presence of 30 mM CaCl2. (D) Currents of a brush-YFP injected oocyte in the presence of 30 mM CaCl2 (left) or 60 mM KCl (right). (E) Current-voltage relations of BRUSH-YFP (▽, n = 8), brush-YFP (●, n = 14), and oocytes injected with water or YFP (▲, n = 16). (F) Mean relative current-voltage relations (n = 4) in the presence of 30 mM CaCl2 (●) and 60 mM KCl (○) ±standard deviations. Currents were normalized to the value at −120 mV in the presence of 30 mM CaCl2. Scale bars (A,B) represent 250 µm. Image published in: Chiasson DM et al. (2017) © 2017, Chiasson et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |