XB-IMG-170768
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Figure S1. Anatomical positions for spinal cord transection and grafting approaches. A-C, Spinal cord (SC) transection (red scissor and dashed line) is performed at the mid-point between hindlimbs and forelimbs (black dashed lines), as indicated for (A) R-, (B) NR56- and (C) NR66-stages. In control groups (sham operation) an incision in skin and muscle is performed at the same level, without injuring the SC. Caudal portion of the SC is isolated from the transection site to (A) the mid-point of the tail in R-stage, (B) the beginning of the cloaca (Vent tube) in NR56-stage and (C) the end of the spinal cord in NR66-stage animals. D, SC grafting is performed by transections (red scissors and dashed lines) of donor and host in the mid-point between limb buds and in the forelimb buds. SC isolation is performed at the same sites. Image published in: Tapia VS et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |