XB-IMG-170772
Xenbase Image ID: 170772
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Figure S5. Characterization of Tg(HS:caSTAT3GR) transgenic tadpoles. A. Transgenic
construct used in this study. caSTAT3GR was inserted in an HGEM construct by HindIII
and BssHII restriction enzymes. B-E, F1 transgenic animals and their control siblings were
raised to stage 50, heat shocked once and incubated with dexamethasone during 2 days. B,
RT-qPCR analysis shows gr up-regulation in the central nervous system (CNS) 6 hours and
1 day post-treatment C, Western blot analysis shows presence of caSTAT3GR protein (red
arrow, GR) in comparison to total STAT3 (black arrows, α and β isoforms) at 8 hours posttreatment
in the CNS and in the tail. D, RT-qPCR analyses shows socs3 up-regulation in
the CNS after treatment. E, Tadpoles expressing caSTAT3GR had a reduced survival rate
in comparison with their controls siblings. For RT-qPCR analyses, one sample t-test with
95% confidence was performed to determine a significant difference (* symbol) from
control tadpoles, using at least 3 independent replicates. Image published in: Tapia VS et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |