Xenbase Image ID: 170837

Figure 5. ICL Structure and Repair Efficiency (A) Structures of normal B-form DNA (PDB: 1BNA) and DNA duplexes containing trimethylene 5′ GpC-ICL (PDB: 1LUH) and 5′ CpG-ICL (PDB: 2KNK) lesions. (B) Plasmids containing single trimethylene GpC or CpG-ICL were incubated in HSS, and soluble extracts were analyzed by WB. (C) Quantification of ICL repair of trimethylene GpC and CpG plasmids at the indicated time points. Results represent mean ± SEM from n = 4 independent experiments. (D) Quantification of ICL repair of trimethylene GpC and CpG plasmids in mock- and MSH2-depleted HSS at 3 hr. Results represent mean ± SEM from n = 4 independent experiments. (E) Quantification of ICL repair of trimethylene GpC and NM-like ICL plasmid using TaqMan qPCR reagent at the indicated time points. Results represent mean ± SEM from n = 3 independent experiments. See also Figure S5. Image published in: Kato N et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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