Xenbase Image ID: 170841

Figure S3. Nicking sites on the ICL plasmid and purification of MutLa, Related to Figure 3 (A) Sequence surrounding the trimethylene GpC-ICL lesion, and the specific locations of near (top) and far (bottom) nicking sites used in Figure 3A,B. (B) Schematic of X.l. PMS2. The domain structure of this protein and the locations of mutations used in the study are illustrated. (C) The sequence of the conserved catalytic nuclease domain in PMS2 is illustrated for several species. The amino acid substitution used in this study is indicated in red. (D) Purified MutLaWT and MutLan.d. complex stained with Coomassie blue. (E) Plasmid pull-down assay of SJG-136 treated plasmids in MLH1-depleted extract (left), or HSS supplemented with MutLaWT and MutLan.d. (right). Plasmid-bound proteins were analyzed by Western blot. Image published in: Kato N et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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