Xenbase Image ID: 170842

Figure S4. RPA is required for RIR, Related to Figure 4 (A) Schematic of X.l. EXO1. The domain structure of this protein and the locations of mutations used in the study are illustrated. (B) Control or SJG-136 ICL oligonucleotide duplexes run on a denaturing polyacrylamide gel and stained with SYBR Gold. (C) RPA immunodepletion. Mock- and RPA-depleted HSS was analyzed by Western blot to demonstrate depletion of RPA. (D) Quantification of ICL repair in Mock-, RPA-depleted, or RPA-depleted HSS supplemented with recombinant RPAWT at 3 hrs. Results represent mean ± SEM of n=4 independent experiments. (E) Purified RPAWT stained with Coomassie blue. (F) Plasmid pull-down assay of SJG-136 treated plasmids in Mock- or RPA-depleted HSS. Plasmid bound proteins were analyzed by Western blot using the indicated antibodies. Image published in: Kato N et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page