Xenbase Image ID: 171105

Fig. 1. Respiratory competence ofXenopusendoderm is restricted by the early gastrula stage. (A) Experimental diagram of the Xenopus respiratory competence assay. Bisected early gastrula stage (NF10.25) Xenopus embryos assayed by in-situ hybridization show the expression of sox17α throughout the definitive endoderm (DE), hhex in anterior endoderm (AE) and ventx2 in posterior endoderm (PE). DE, AE, or PE explants were dissected at NF10.25, cultured in isolation until NF14, treated +/− 50 nM RA from NF14-25, and then in 3.5 μM Bio + 50 ng/mL BMP4 from NF25-38, at which time they were fixed and analyzed by in-situ hybridization for the respiratory endoderm markers nkx2-1(B-G) and sftpc(H-M). Image published in: Rankin SA et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 10.25 endoderm hhex.L hex, tHex, XHex X. laevis Throughout NF stage 10.25 anterior endoderm ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 10.25 posterior endoderm Image source: Published Larger Image Printer Friendly View

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