XB-IMG-171124
Xenbase Image ID: 171124
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Fig. 3. Antisense dullard morpholino oligonucleotide (dullard Mo) blocked dullard mRNA function and injection of the dullard Mo caused failure of
neural tube closure and disorganization ofneural tissue. (A) Dullard mRNA (200 pg), injected embryos show white mottled aspects, characteristic of
apoptosis. (B) This effect was blocked by co-injection of dullard Mo (40 ng). (C–Q) Dullard Mo (40 ng/embryo) was injected in dorsal animal
blastomeres of8-cell stage embryos and the embryos raised until stage 24 or 35. (C) Control embryo at stage 24. (D) Dullard Mo (40 ng), injected
embryo at stage 24 shows failure ofneural tube closure. (E, F) Transverse sections ofstage 24 control and dullard Mo-injected embryos, respectively.
In the dullard Mo-injected embryo, the neural tube fails to close while other structures are normal. (G) Control embryo at stage 35. (H) Dullard Mo
(40 ng), injected embryo at stage 35 shows a reduction ofhead development and poor axis formation. (I) These effects were rescued by co-injection of
d5n-dullard mRNA. (J–Q) Transverse sections ofthe control (J–M) or dullard Mo-injected (N–Q) embryo at stage 35 at the sites indicated with small
letters on the whole embryos. The transverse section through the forebrain of a dullard Mo-injected embryo compared to that ofa control embryo
shows the absence ofhead structures (J, N). The transverse section through the midbrain ofa dullard Mo-injected embryo compared to that ofa
control embryo shows no or poor eye formation and disorganization of the midbrain (K, O). The transverse sections through the hindbrain and
spinal cord ofthe dullard Mo-injected embryo compared to that ofa control embryo show disorganization ofbrain and neural tube, while other
structures around them appear unaffected (L, M, P, Q). Image published in: Satow R et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |