Xenbase Image ID: 171125

Fig. 4. Microinjection of dullard Mo suppressed expression of neural markers. Control morpholino oligonucleotide (A, C, E, G, I, K) or dullard Mo (B, D, F, H, J, L) (40 ng) together with lacZ mRNA (200 pg) as a tracer was injected into a dorsal animal blastomere of8-cell embryos. Embryos were harvested at the early tail-bud stage (A–D) or the neurula stage (remaining panels) and analyzed by whole mount in situ hybridization with the indicated probes. In the dullard Mo-injected side ofthe embryo, expression ofthese markers is downregulated (B, D, F, H, J, L), while no change is observed in the control morpholino oligonucleotide-injected embryos (A, C, E, G, I, K). (A and B) lateral view, (C–F) dorsal view, (G–L) anterior view. Image published in: Satow R et al. (2002) Copyright © 2002. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 28 spinal cord nerve tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 18 neural plate neuron ncam1.L n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 28 spinal cord central nervous system brain egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 18 presumptive rhombomere otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 18 pre-chordal neural plate anterior en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 18 presumptive midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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