Xenbase Image ID: 171165
Fig. 2. DVL2 is a substrate of PGAM5.
(A) Knockdown of PGAM5 in HEK 293T cells was
sufficient to induce hyperphosphorylation of DVL2.
(B) Overexpressed HA-DVL2 yielded two distinct
bands on western blots. Co-expression of PGAM5-Flag
visibly diminished the slower migrating,
hyperphosphorylated band, whereas the phosphatasedeficient
mutant PGAM5-H105A-Flag did not affect
electrophoretic mobility of HA-DVL2. (C) Cell lysates
were incubated with recombinant GST (control) or
GST-tagged PGAM5δ (lacking the N-terminal
mitochondrial targeting sequence) for 30 min. In the
presence of recombinant GST-PGAM5δ, DVL2 was
gradually dephosphorylated. For B and C, intensities
have been quantified and the ratio between the
hyperphosphorylated band (a) and the faster-migrating
band (b) is provided below the corresponding blots.
Image published in: Rauschenberger V et al. (2017)
Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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