XB-IMG-171165
Xenbase Image ID: 171165
|
Fig. 2. DVL2 is a substrate of PGAM5.
(A) Knockdown of PGAM5 in HEK 293T cells was
sufficient to induce hyperphosphorylation of DVL2.
(B) Overexpressed HA-DVL2 yielded two distinct
bands on western blots. Co-expression of PGAM5-Flag
visibly diminished the slower migrating,
hyperphosphorylated band, whereas the phosphatasedeficient
mutant PGAM5-H105A-Flag did not affect
electrophoretic mobility of HA-DVL2. (C) Cell lysates
were incubated with recombinant GST (control) or
GST-tagged PGAM5δ (lacking the N-terminal
mitochondrial targeting sequence) for 30 min. In the
presence of recombinant GST-PGAM5δ, DVL2 was
gradually dephosphorylated. For B and C, intensities
have been quantified and the ratio between the
hyperphosphorylated band (a) and the faster-migrating
band (b) is provided below the corresponding blots. Image published in: Rauschenberger V et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |