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Xenbase Image ID: 171167

Fig. 4. Pgam5 modulates Wnt/β-Catenin signaling in Xenopus laevis. Embryos were injected with 0.4 pmol Pgam5 MO1 or control MO in both blastomeres at the two-cell stage. (A) The Pgam5 MO1 phenotypes are rescued by co-injection of either 100 pg MO-insensitve Xenopus pgam5 RNA or 10 pg dkk1-gfp DNA, as indicated. Scale bar: 500 μm. The frequencies of the indicated phenotypes from at least three independent experiments with the indicated total numbers of embryos are summarized in the graph (**P<0.01, χ2-test). (B) Pgam5 depletion resulted in overall elevated levels of active dephosphorylated β-Catenin (ABC) and hyperphosphorylation of Dvl2 in NF stage 11.5 embryos. Band intensities of ABC, β-Catenin (BC), hyperphosphorylated and dephosphorylated Dvl2 (a and b, respectively) have been quantified and the intensity ratios for ABC/BC and Dvl2 a/b are given below the respective blots. Pgam5 knockdown resulted in upregulation of endogenous Wnt/β-Catenin target genes. (C,D) Relative expression of the indicated genes from four independent experiments (data are average ±s.d.). Statistically significant deviations are indicated by asterisks [**P<0.01, *P<0.05, (*)P<0.1, t-test for the hypothesis of the mean]. (E) Knockdown of PGAM5 also strongly enhanced responsiveness of HEK 293T cells to WNT3A stimulation, as determined by TOP-Flash reporter gene assays.

Image published in: Rauschenberger V et al. (2017)

Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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