XB-IMG-171168
Xenbase Image ID: 171168
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Fig. 5. Pgam5 is required for anterior inhibition of Wnt/β-Catenin signaling and ectodermal anterior-posterior patterning. (A) The prospective neural plate
was explanted from embryos injected with 0.4 pmol Pgam5 MO1 or controlMOat NF stage 11.5 and dissected into anterior, mid and posterior thirds as indicated in
the schematic. Active dephosphorylated β-Catenin (ABC) was detected in an anterior to posterior gradient in lysates from control dorsal explants. The blots
show one representative experiment; data in the graph are average ratios±s.d. of ABC/β-Catenin from four independent experiments (*P<0.05, separate variances
t-test). (B) Embryos were injected into one dorsal blastomere at the four-cell stage with 0.4 pmol Pgam5 MO1 or control MO, pCS2+ lacZ as lineage tracer and
PGAM5 RNAor PGAM5-H105ARNAas indicated.Embryoswere stained for β-galactosidase to identify the injected side (asterisk) and analyzed for the expression
of krox20 and otx2 at NF stage 21 by in situ hybridization. Images show representative embryos; the corresponding pattern is schematically shown in the upper right
corner. The graph summarizes the results from five independent experiments (**P<0.01, *P<0.05, n.s. not significant, Wilcoxon-Rank-Sum test). Image published in: Rauschenberger V et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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