XB-IMG-171169
Xenbase Image ID: 171169
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Fig. 6. PGAM5 overexpression inhibits Wnt/β-Catenin signaling in Xenopus embryos and human cells. (A) Secondary body axes were induced in Xenopus
laevis embryos by injection of 5 pg wnt8 RNA and 100 pg PGAM5 or PGAM5-H105A RNAwere co-injected as indicated into both ventral blastomeres at the fourcell
stage. Images show representative examples of embryos; filled arrowheads indicate primary axes, open arrowheads indicate secondary axes. The graph
shows the frequency of complete and partial axis duplication from five independent experiments. (B) Phenotypes of embryos injected with 200 pg egfp, PGAM5
RNA or PGAM5-H105A RNA (H105A RNA) in both blastomeres at the two-cell stage. Arrowheads indicate the dorsal fin and somites. The frequencies of the
indicated phenotypes from at least three independent experiments are summarized in the graph. The statistical significance of deviations in A and B was
calculated using theWilcoxon Rank Sum test; **P<0.01, *P<0.05. (C) Pgam5 overexpression reduced nuclear β-Catenin levels in Xenopus embryonic fibroblasts
(XTC cells). Cells were transfected as indicated, stimulated with WNT3A-conditioned medium for 1 h and immunostained for endogenous β-Catenin. Scale bar:
50 μm. The graph summarizes nuclear β-Catenin intensity of at least 20 GFP-positive and GFP-negative cells (**P<0.01, separate variance t-test). Image published in: Rauschenberger V et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |