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Xenbase Image ID: 171178

Supplementary Figure 7 (A) Expression of overexpressed proteins corresponding to Figure 7A was confirmed by Western Blot. Filled arrowheads indicate endogenous proteins, open arrowheads indicate overexpressed proteins. (B) Xenopus Pgam5 antagonized TOP-Flash activation by constitutively-active LRP6, Dvl2, β-Catenin or stabilized β-Catenin 4ST/A-Myc. The graph shows average ± SD from at least three independent experiments. Statistical significance of deviations was calculated using the t-test for the hypothesis of the mean (* p-value < 0.05). Expression of overexpressed proteins was confirmed by Western Blot; corresponding blots are provided below the respective columns. (C) Overexpression of PGAM5 in SW480 colon carcinoma cells suppressed constitutive Wnt/β-Catenin activity; the phosphatase-inactive mutant PGAM5-H105A had no effect. Overexpression of AXIN downregulated TOP-Flash activity comparable to PGAM5. TOP-Flash values were normalized to β-Galactosidase. (D) Secondary body axes were induced in Xenopus laevis embryos by injection of 400 pg β-catenin RNA and 100 pg pgam5 RNA was co-injected as indicated into both ventral blastomeres at the four-cell stage. The graph shows the frequency of axis duplication from four independent experiments; statistical significance of deviations was calculated using the χ2-test; ** p-value < 0.01. Images show representative examples of embryos; arrows indicate neural tubes in the primary and – if present – in the secondary axis.

Image published in: Rauschenberger V et al. (2017)

Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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