XB-IMG-171178
Xenbase Image ID: 171178
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Supplementary Figure 7
(A) Expression of overexpressed proteins corresponding to Figure 7A was confirmed by Western Blot. Filled
arrowheads indicate endogenous proteins, open arrowheads indicate overexpressed proteins. (B) Xenopus
Pgam5 antagonized TOP-Flash activation by constitutively-active LRP6, Dvl2, β-Catenin or stabilized β-Catenin
4ST/A-Myc. The graph shows average ± SD from at least three independent experiments. Statistical significance
of deviations was calculated using the t-test for the hypothesis of the mean (* p-value < 0.05). Expression of
overexpressed proteins was confirmed by Western Blot; corresponding blots are provided below the respective
columns. (C) Overexpression of PGAM5 in SW480 colon carcinoma cells suppressed constitutive Wnt/β-Catenin
activity; the phosphatase-inactive mutant PGAM5-H105A had no effect. Overexpression of AXIN downregulated
TOP-Flash activity comparable to PGAM5. TOP-Flash values were normalized to β-Galactosidase. (D) Secondary
body axes were induced in Xenopus laevis embryos by injection of 400 pg β-catenin RNA and 100 pg pgam5 RNA
was co-injected as indicated into both ventral blastomeres at the four-cell stage. The graph shows the frequency
of axis duplication from four independent experiments; statistical significance of deviations was calculated using
the χ2-test; ** p-value < 0.01. Images show representative examples of embryos; arrows indicate neural tubes in
the primary and – if present – in the secondary axis. Image published in: Rauschenberger V et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |