Xenbase Image ID: 171206

Fig. S2. Stimulation of voltage-gated Na+ channels does not affect Ca2+ transients or Gli activity in the neural plate. (A) Two-cell-stage embryos were injected with the genetically encoded Ca2+ sensor, GCaMP6s, and time-lapse imaged at neural plate stages (stage 14, 16.25 hpf) for 30 min at 0.2 Hz in the absence or presence of 1 μM veratridine, voltage-gated Na+ channel agonist. Graph shows mean ± SEM percentage of neural plate cells exhibiting Ca2+ transients in the presence or absence of veratridine; n = 5. (B) Neural plates from embryos expressing 8GLI-luciferase were incubated for 8 h with the indicated agents and processed for luciferase activity measurements. Graph shows mean ± SEM percentage of normalized luciferase intensity compared with control (incubated with vehicle only); n = 5; *P < 0.05; ns: not significant. Image published in: Belgacem YH and Borodinsky LN (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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