XB-IMG-171211
Xenbase Image ID: 171211
|
Fig. S7. Shh mimics PKA-induced regulation of Gli2 subcellular localization in spinal cord cells. Spinal cords from mGli2-GFP–expressing 21-hpf embryos were
dissected, and cells were dissociated and plated. After 2 h, cultures were time-lapse imaged under a confocal microscope for a 1-h period every 15 s. (A) A
representative region of a field of view of imaged cells that were fixed and labeled with the nuclear marker DAPI (in red) after imaging. (B) Orthogonal view of
the z-stack projection of an imaged mGli2-GFP–expressing cell showing significant overlap between Gli2-GFP signal and DAPI. (C) Representative examples of
imaged cells under indicated treatments. Contour of imaged cells and nuclei are indicated with dashed and solid lines, respectively. Color scale bar represents
fluorescence intensity increasing from purple to red. Traces represent changes in nuclear (black) and cytosolic (gray) fluorescence over time for the given
examples. n ≥ 27 cells per condition. (Scale bars: 10 μm.) Image published in: Belgacem YH and Borodinsky LN (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |