XB-IMG-171219
Xenbase Image ID: 171219
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Figure 2.
Depletion of Rapgef5 Impairs Canonical Wnt Signaling
(A) Depletion of Rapgef5 using MOs or CRISPR impairs foxj1 and xnr3 expression in the dorsal blastopore lip of stage 10 embryos.
(B) Simplified schematic of the canonical Wnt signaling pathway. Left side: in the absence of Wnt ligand, a destruction complex containing Axin and Gsk3 phosphorylates β-catenin, which marks it for cytoplasmic destruction. Right side: once the pathway is activated by Wnt ligand binding to Frizzled and Lrp receptors, phosphorylation of β-catenin by GSK3 is inhibited allowing β-catenin to accumulate in the cytoplasm and translocate into the nucleus to initiate transcription of Wnt target genes.
(C and D) Levels of total and active β-catenin protein are essentially unchanged in Rapgef5-depleted embryos at stage 9 as assayed by western blot. However, both forms of β-catenin are reduced at stages 10 and 12. Note that levels of active β-catenin are much more severely affected. Conversely, overexpression of human RAPGEF5 mRNA results in a mild increase in total β-catenin levels and a more pronounced increase in active β-catenin levels.
∗p < 0.05, ∗∗∗p < 0.005. See also Figure S3. Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
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