Xenbase Image ID: 171223

Figure 6. Depletion of Rapgef5 Impairs Nuclear Translocation of Endogenous β-Catenin (A) Nuclear/cytoplasmic fractionation reveals that levels of endogenous β-catenin are reduced in the nuclei of Rapgef5 morphants, while cytoplasmic levels are essentially normal. (B) BIO treatment does not rescue the nuclear localization of endogenous β-catenin. (C) Quantification of average β-catenin levels in nuclear and cytoplasmic fractions of control and morphant embryos with or without BIO treatment (error bars represent SEM). All nuclear treatments (black bars) are relativized to the BIO-untreated nuclear control, and all cytoplasmic treatments (gray bars) are relativized to the BIO-untreated cytoplasmic control. Note the reduction of nuclear-localized β-catenin in Rapgef5-depleted embryos with or without BIO treatment. The BIO-treated rapgef5 morphants have less nuclear-localized β-catenin than BIO-untreated controls (UCs), despite having higher levels of cytoplasmic β-catenin. The cellular compartment markers (H3 for nuclear fraction and β-actin for cytoplasmic fraction) displayed in (A and B) are overexposed to demonstrate the degree of purification. For quantitation of β-catenin (C), shorter exposures of the gel (unsaturated) were used in which these markers serve to normalize the amount of protein loaded for each compartment. ∗p < 0.05, ∗∗p < 0.01. Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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