Xenbase Image ID: 171224

Figure 7. Active Raps and β-Catenin Interact (A) The GFP-RBDRalGDS active Rap sensor reveals the presence of active Raps in the nucleus and plasma membrane of dorsal blastopore lip cells at stage 10. (B) Rap1 protein co-immunoprecipitates with β-catenin. IP, immunoprecipitate. (C) Pull-down of active Rap proteins from stage 10 Xenopus embryos using the RalGDS sensor. β-Catenin co-immunoprecipitates with active Raps demonstrating an interaction. (D) Injection of CA Rap1b mRNA can partially rescue loss of foxj1 expression in rapgef5 morphants, while DN Rap1b mRNA does not. ∗∗∗p < 0.0001. See also Figure S7. Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxj1.L foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 X. laevis Throughout NF stage 10 dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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