XB-IMG-171226
Xenbase Image ID: 171226
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Figure S2. Related to Fig. 1; Depletion of Rapgef5 protein. (A) Western blot
demonstrating the efficacy of the R5 MOATG, R5 MOSplice, and CRISPR mediated
Rapgef5 depletion in stage 10 Xenopus embryos. Overexpression of human
RAPGEF5 mRNA increases protein levels. (B) Timeline of Rapgef5 depletion. Rapgef5
protein levels were unaltered in morphants at late blastula stages (st 8 and 9) but
reduced at stage 10 and 12. The graph represents fold changes in Rapgef5 protein
levels in control and depleted embryos relative to the stage 8 uninjected controls. Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |