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Figure S5. Related to Fig. 6; Developmental expression of Rapgef5 and Rap
genes. In situ hybridization reveals rapgef5 mRNA is present from the earliest stages
of development. It is detected in the animal pole at the 2 cell and 8 cell stages. In the
stage 10 embryos expression appears diffuse around the blastopore. At stage 14
transcripts are detected in the anterior neural folds and forming GRP. rapgef5
expression is restricted to the anterior neural tube, brain, pharyngeal arches and
cranial placodes at stage 28. rap1A, rap2A and rap2B are all expressed in the 4 cell
embryo, in the gastrulating stage 10 embryo and in the GRP at stage 16. Expression
of all three genes is also strong in the neural folds at stage 16, while rap2B also
displays a speckled pattern on the lateral ectoderm. All three are expressed in the
neural tube, brain, eye and pharyngeal arches at stage 28.
Image published in: Griffin JN et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|
| rapgef5 | | X.tropicalis | Throughout NF stage 2 (2-cell) | blastomere
| | rapgef5 | | X.tropicalis | Throughout NF stage 4 (8-cell) | animal blastomere
| | rapgef5 | | X.tropicalis | Throughout NF stage 10 | | | rapgef5 | | X.tropicalis | Throughout NF stage 14 | gastrocoel roof plate
pre-chordal neural plate border
| | rapgef5 | | X.tropicalis | Throughout NF stage 28 | anterodorsal lateral line placode
epibranchial placode
glossopharyngeal epibranchial placode
facial epibranchial placode
central nervous system
spinal cord
pharyngeal arch
branchial arch
otic vesicle
| | rap1a | rap1, krev1, krev-1 | X.tropicalis | Throughout NF stage 3 (4-cell) | blastomere
| | rap1a | rap1, krev1, krev-1 | X.tropicalis | Throughout NF stage 10 | | | rap1a | rap1, krev1, krev-1 | X.tropicalis | Throughout NF stage 16 | gastrocoel roof plate
| | rap1a | rap1, krev1, krev-1 | X.tropicalis | Throughout NF stage 28 | central nervous system
spinal cord
eye
brain
pharyngeal arch
mandibular arch
hyoid arch
branchial arch
somite
| | rap2a | krev, rap2, k-rev, rbbp-30 | X.tropicalis | Throughout NF stage 3 (4-cell) | marginal zone
blastomere
| | rap2a | krev, rap2, k-rev, rbbp-30 | X.tropicalis | Throughout NF stage 16 | gastrocoel roof plate
neural plate
neural fold
| | rap2a | krev, rap2, k-rev, rbbp-30 | X.tropicalis | Throughout NF stage 28 | eye
brain
forebrain
midbrain
hindbrain
pharyngeal arch
mandibular arch
hyoid arch
central nervous system
spinal cord
branchial arch
otic vesicle
| | rap2b | | X.tropicalis | Throughout NF stage 3 (4-cell) | blastomere
| | rap2b | | X.tropicalis | Throughout NF stage 10 | marginal zone
| | rap2b | | X.tropicalis | Throughout NF stage 16 | gastrocoel roof plate
neural crest
cranial neural crest
non-neural ectoderm
| | rap2b | | X.tropicalis | Throughout NF stage 28 | pharyngeal arch
mandibular arch
hyoid arch
branchial arch
posterior
|
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