Xenbase Image ID: 171230

Figure S6. Related to Fig. 6; The subcellular localization of Rapgef5 and Rap proteins. (A1) Rapgef5 is localized to the nuclei in a section of a stage 10 Xenopus embryo. (A2 - 7) Rapgef5 is detected specifically in the nuclei of sectioned paraffin embedded stage 28 Xenopus embryos but is reduced in morphants (Compare A2 and A3, magenta Rapgef5, blue DAPI). The lines on the schematic in A4 represent the plane of section shown in A4 and A5. A6 and A7 are higher magnification of the white boxes in A4 and A5, respectively. (B, C) Rapgef5 localizes specifically to the nuclei of mouse MEFs and human RPE cells. (D) With the exception of Rap1A all rap proteins localize both to the plasma membrane and nucleus as assayed by injection of GFP/RFP tagged constructs or by immunofluorescence. Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rapgef5 X. tropicalis Sometime during NF stage 10 to NF stage 28 whole organism nucleus rap2a k-rev, krev, LOC100505442, rap2, rbbp-30 X. tropicalis Sometime during NF stage 8 to NF stage 12 whole organism nucleus Image source: Published Larger Image Printer Friendly View

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