Xenbase Image ID: 171231

Figure S7. Related to Fig. 7; A Rap1 mediated transport system. (A) Validation of the CA (constitutively active) and DN (dominant negative) Rap1 constructs. RBDRalGDS selectively pulls down endogenous active RAPs, as well as the GFP tagged CA, but not the DN Rap1b mutant. (B) While the GFP tagged CA and DN Rap1 constructs can be equally detected in the total lysate, the level of the CA form is greatly reduced in the first flow through from the pull down columns, reflecting the binding of CA Rap1 to RBDRalGDS and its retention in the column. (C) Injection of CA Rap1a or Rap1b leads to an increase in total β-catenin protein levels relative to controls, while injection of the DN forms causes a decrease in total β-catenin levels. Injection of Rap2a CA or DN did not reveal a differential effect on β-catenin. (D) Proposed model for Rapgef5s role in nuclear localization of β-catenin. Rapgef5 maintains nuclear Raps in their active GTP bound confirmation, which is required for 1) – the nuclear import of β- catenin or 2) – retention of β-catenin within the nuclear compartment. Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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