Xenbase Image ID: 171268

Supplementary Figure 7. Nodal/Activin induces neural tissue in Xenopus animal cap assays. a, RT-qPCR from animal caps treated from stage 11 to 15 with Nodal. Bars represent SEM of 5 independent experiments, represents undetectable (background) gene expression levels. Statistical analysis has been performed using Paired t-test, * p<0.05; ** p<0.005. b, RT-qPCR from animal caps injected with GR-t-Smad2, explanted at early gastrula stage and induced or not with dexamethasone at stage 10 or 11. Uninjected animal caps were treated with ethanol and served as reference. Bars represent SEM of 4 independent experiments, except for chd and otx2 for which they represent 2 independent experiments. Statistical analysis has been performed using Two-way ANOVA followed by Bonferroni post-test; * p<0.05; ** p<0.005; ***p<0.001. c, RT-qPCR analyses of sox2 and foxD5 expression in animal caps treated with cycloheximide, Activin, or both. Error bars indicate SEM. Image published in: Le Petillon Y et al. (2017) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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