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Figure 3. Mitochondrial Inhibitors Malonate, Antimycin A, and Sodium Azide Impair ROS Production after Activation(A) NOX inhibitors 10 μM DPI or 1 mM apocynin had no effect on HyPer ratio in mature oocytes from HyPer transgenic females after pricking. n = 29–42, two-way ANOVA and Tukey’s post hoc tests.(B–F) HyPer ratio on oocytes treated with 1 μM rotenone (n = 36; B), 5 mM malonate (n = 45; p < 0.0001, compared to control at each time point, two-way ANOVA and Sidak post hoc tests; C), 10 μM antimycin A (n = 46; p < 0.0001, compared to control at 40 and 60 min, two-way ANOVA and Sidak post hoc tests; D), 1 mM sodium azide (n = 25; p < 0.0001, compared to control at 40 and 60 min, two-way ANOVA and Sidak post hoc tests; E), and 6 μM oligomycin (n = 42; F).(G) Ca2+ wave was measured by fluorescent intensity of R-GECO in laser-activated mature oocytes. Ca2+ wave was not affected by any of the mitochondrial inhibitors. Each treatment, n = 6; two independent experiments; two-way ANOVA and Tukey’s post hoc tests. See also Movie S7.(H) HyPer ratio on oocytes treated with 2 μM FCCP. n = 36; p < 0.0001, compared to control at 40 and 60 min, two-way ANOVA and Sidak post hoc tests.Data for (A)–(F) and (H) are from three or four independent experiments. Error bars represent mean ± SEM; ns, not significant; ∗∗∗∗p < 0.0001. Image published in: Han Y et al. (2018) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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