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Figure 6. Removal of Inhibitors Restores Cell Division and ROS Production(A) Embryos were treated with inhibitors, 10 mM malonate, 3 mM sodium azide, and 1 μM rotenone, for 40 min until cells stopped dividing (left column), and then they were transferred to a dish containing fresh medium without inhibitors. Embryos transferred after treatment with malonate and azide retrieved cell division and divided to 8- to 16-cell stages (middle), but not in medium with inhibitors (right). Pictures are representative of three independent experiments.(B) Fertilized transgenic embryos expressing HyPer were treated with inhibitors for 40 min and imaged shown as 0 min. Then embryos were transferred to fresh medium without inhibitors, and imaged at 40 and 70 min (n = 34–36; three independent experiments).Error bars represent mean ± SD. See also Movie S9. Image published in: Han Y et al. (2018) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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