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Figure 7. MarvelD3 is required for neural crest induction. Expression of the neural crest markers twist and snai2/slug during neural crest induction (stage 15) was detected by WISH in animal caps explants injected with chd mRNA + wnt8 mRNA alone (A,B) and co-injected with control (C,D) or MD3AB morpholinos (E,F); scale bar, 500 μm. Neural crest induction phenotype was quantified in animal caps and classified as positive (dark green), weak (light green) and negative (red) for twist (G) and snai2/slug (H) expression. Student t-test p values and the number of animal caps analysed are indicated. Image published in: Vacca B et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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