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Figure 9. Rescue of neural crest formation by small molecule inhibitor for JNK in MarvelD3 depleted embryos. Neural crest induction (stage 15) was analysed by WISH against twist in control and MD3AB morpholino-injected embryos (A–D; red asterisk, injected side of the embryo with β-galactosidase staining) and against snai2/slug in animal caps explants co-injected with chd mRNA + wnt8 mRNA + control morpholino or chd mRNA + wnt8 mRNA + MD3AB morpholinos. Both embryos and animal caps were treated from stage 11 to 15 with DMSO or 0,5 μg.ml−1 SP600125, a JNK inhibitor (E–H). Neural crest induction was quantified according to twist or snai2/slug expression in embryos (I) and animal caps (J), respectively. Mann-Whitney test p values and the number of embryos or animal caps analysed are indicated; red asterisk, injected side of the embryo with light blue β-galactosidase staining. (I) blue bar, normal neural crest; red bar, reduced neural crest; (J) dark green bar, positive; light green bar, weak; red bar, negative snai2/slug staining. + , embryos or animal caps positive or weak for twist or snai2/slug expression; -, embryos or animal caps negative for twist or snai2/slug expression. Scale bars, 500 μm. Image published in: Vacca B et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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