Xenbase Image ID: 171378

Figure 10. JNK pathway inhibition is required for neural crest formation in MarvelD3 knockdown embryos. Neural crest induction (stage 15) was analysed and quantified by WISH against twist in control and MD3AB morpholino embryos co-injected with constitutively active-jnk (ca-jnk) or dominant negative-jnk mRNA (dn-jnk) (A–D and I) and against snai2/slug in animal caps co-injected with chd mRNA + wnt8 mRNA + control morpholino or chd mRNA + wnt8 mRNA + MD3AB morpholinos and with constitutively active-jnk or dominant negative-jnk mRNA (E–H and J). Mann-Whitney test p values and the number of embryos or animal caps analysed are indicated; red asterisk, injected side of the embryo with light blue β-galactosidase staining. (I) blue bar, normal neural crest; red bar, reduced neural crest; (J) dark green bar, positive; light green bar, weak; red bar, negative snai2/slug staining. + , embryos or animal caps positive or weak for twist or snai2/slug expression; -, embryos or animal caps negative for twist or snai2/slug expression. Scale bars, 500 μm. Image published in: Vacca B et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 15 neural crest neuroectoderm Image source: Published Larger Image Printer Friendly View

Return to previous page