Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-IMG-171427

Xenbase Image ID: 171427


Fig. 2. ADMP provides dorsal signal required in Spemann's organizer at the onset of gastrulation. ADMP knockdown was induced by ADMPMO injection (7 ng/embryo) and subjected to analysis of changes in gene expression. a, b qPCR analysis of the effects on dorsal and ventral gene expression during early gastrulation (stage 10–10.25) (a) and later during early/mid-gastrula (stage 10.25–10.5) (b). c–n Effect of ADMPMO injection on the domains of chordin (c–f), gsc (g–j), and ADMP (k–n) expression. Embryos injected with ADMPMO (4 ng) either dorsally or ventrally were fixed at the onset of gastrulation. f, j, n The arc of the domain of expression was measured, and the size of the domain of expression relative to control embryos is shown (%). o–r The specificity of the ADMPMO was further tested in rescue experiments. Embryos were injected dorsally (turquoise) with ADMPMO (3.4 ng/embryo) alone or with increasing amounts of RNA encoding the zebrafish (ZF) ADMP protein (10 or 20 pg RNA/embryo). o–q In situ hybridization analysis of the changes in the chordin expression domain in control (o), ADMPMO injected (p), and embryos co-injected with ADMPMO and zebrafish ADMP mRNA (20 pg/embryo) (q). r Summary of the quantitation of the changes in the size of the chordin expression domain. Chordin analysis: control, n = 81; ADMPMO dorsal, n = 48; ADMPMO ventral, n = 33, gsc analysis: control, n = 19; ADMPMO dorsal, n = 20; ADMPMO ventral, n = 15, ADMP analysis: control, n = 20; ADMPMO dorsal, n = 18; ADMPMO ventral, n = 14; and chordin rescue analysis: control, n = 25; ADMPMO, n = 25; ADMPMO + ZF ADMP 10, n = 19; ADMPMO + ZF ADMP 20, n = 25. p values were calculated compared to controls (a, b, f, j, n) except in the rescue experiments that were compared to the ADMPMO sample (r). Statistical test; Dunnett’s (ANOVA) multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant

Image published in: Leibovich A et al. (2018)

Image downloaded from an Open Access article in PubMed Central. © Fainsod et al. 2018

GeneSynonymsSpeciesStage(s)Tissue
chrd.1.Lchd, chordin, X-chordin, chrdX.laevisThroughout NF stage 10 to NF stage 10.25dorsal marginal zone
admp.LX.laevisThroughout NF stage 10 to NF stage 10.25dorsal marginal zone
gsc.LXgsc, goosecoid, gsc-a, gsc-bX.laevisThroughout NF stage 10 to NF stage 10.25dorsal marginal zone

Image source: Published

Larger Image
Printer Friendly View

Quality:
Good quality Poor quality
comment [+]
Return to previous page
Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.11.4


Major funding for Xenbase is provided by grant P41 HD064556