XB-IMG-171427
Xenbase Image ID: 171427
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Fig. 2. ADMP provides dorsal signal required in Spemann's organizer at the onset of gastrulation. ADMP knockdown was induced by ADMPMO injection (7Â ng/embryo) and subjected to analysis of changes in gene expression. a, b qPCR analysis of the effects on dorsal and ventral gene expression during early gastrulation (stage 10â10.25) (a) and later during early/mid-gastrula (stage 10.25â10.5) (b). cân Effect of ADMPMO injection on the domains of chordin (câf), gsc (gâj), and ADMP (kân) expression. Embryos injected with ADMPMO (4Â ng) either dorsally or ventrally were fixed at the onset of gastrulation. f, j, n The arc of the domain of expression was measured, and the size of the domain of expression relative to control embryos is shown (%). oâr The specificity of the ADMPMO was further tested in rescue experiments. Embryos were injected dorsally (turquoise) with ADMPMO (3.4Â ng/embryo) alone or with increasing amounts of RNA encoding the zebrafish (ZF)Â ADMP protein (10 or 20Â pg RNA/embryo). oâq In situ hybridization analysis of the changes in the chordin expression domain in control (o), ADMPMO injected (p), and embryos co-injected with ADMPMO and zebrafish ADMP mRNA (20Â pg/embryo) (q). r Summary of the quantitation of the changes in the size of the chordin expression domain. Chordin analysis: control, nâ=â81; ADMPMO dorsal, nâ=â48; ADMPMO ventral, nâ=â33, gsc analysis: control, nâ=â19; ADMPMO dorsal, nâ=â20; ADMPMO ventral, nâ=â15, ADMP analysis: control, nâ=â20; ADMPMO dorsal, nâ=â18; ADMPMO ventral, nâ=â14; and chordin rescue analysis: control, nâ=â25; ADMPMO, nâ=â25; ADMPMOâ+âZF ADMP 10, nâ=â19; ADMPMOâ+âZF ADMP 20, nâ=â25. p values were calculated compared to controls (a, b, f, j, n) except in the rescue experiments that were compared to the ADMPMO sample (r). Statistical test; Dunnettâs (ANOVA) multiple comparisons test. *pâ<â0.05, **pâ<â0.01, ***pâ<â0.001, ****pâ<â0.0001, ns not significant Image published in: Leibovich A et al. (2018) © Fainsod et al. Creative Commons Attribution license
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