XB-IMG-171443
Xenbase Image ID: 171443
|
Figure 1. FGF signaling is required for proper blastula stage gene expression.(A) In situ hybridization examining FGFR4 expression in wildtype Xenopus embryos collected at blastula (stage 9, lateral view, animal pole up), late gastrula (stage 12, dorsal view, anterior up), and mid-neurula (stage 15, dorsal view, anterior up) stages. Expression is seen in the pluripotent cells of the animal hemisphere at blastula stages and in the neural plate and neural crest forming regions at gastrula and neurula states. (B) Animal pole explant assay examining FGFR4 expression. Explants were cultured alongside sibling embryos and collected at blastula (stage 9), late gastrula (stage 12), and mid-neurula (stage 15) stages. (C) In situ hybridization examining Vent2, Id3, Myc, and FoxD3 expression in blastula stage (stage 9) embryos injected with dominant-negative FGFR4 (dnFGFR4). Asterisk denotes injected side, marked by staining of the lineage tracer β-galactosidase (red). Dominant-negative FGFR4 blocks expression of Vent2 and Id3.Figure 1—figure supplement 1. Blocking FGF signaling using dnFGFR4, but not dnFGFR1, leads to a loss in neural crest gene expression at mid-neurula stages.(A–B). In situ hybridization examining FoxD3, Sox9, and Snail2 expression in mid-neurula stage (stage 16) embryos injected with dnFGFR4 (A) or dnFGFR1 (B). Asterisk denotes injected side, marked by staining of the lineage tracer β-galactosidase (red). Blocking FGF signaling using dnFGFR4 causes a loss of FoxD3, Sox9, and Snail2 expression. (C) Quantification of effects on FoxD3 expression in embryos injected with dnFGFR4 or dnFGFR1 scored for loss of neural crest/exclusion of injected cells from the neural crest, normalized to control injections. (ns, not significant; **p<0.01). Image published in: Geary L and LaBonne C (2018) © 2018, Geary et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |