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Figure 4. PI3K/Akt signaling but not MAPK signaling is required for pluripotent blastula cells to transit to a neural progenitor state.(A–B) Animal pole explant assay examining Sox2, Sox3, and Nrp1 expression in Chordin (Chd) induced animal cap explants treated with Meki (RDEA119) (A) or injected with dnPI3K (B). Explants were cultured alongside sibling embryos and collected at early neurula stages (stage 13) for Sox2/3 or late neurula stages (stage 18) for Nrp1. Meki treatment does not affect Chordin-mediated neural induction whereas dnPI3K blocks induction of all three neural markers.Figure 4—figure supplement 1. Blocking PI3K/Akt activation using PI3Ki (L) (LY294) or PI3Ki (W) (Wortmannin) phenocopies the effects of dnPI3K on Chordin-mediated neural induction.(A–C) Animal pole explant assay examining Sox2, Sox3, and Nrp1 expression in Chordin (Chd) induced animal cap explants injected with dnRaf (A) or treated with PI3Ki (L) (B) or PI3Ki (W) (C). Explants were cultured alongside sibling embryos and collected at early neurula stages (stage 13) for Sox2/3 or late neurula stages (stage 18) for Nrp1. PI3Ki (L) and PI3Ki (W) phenocopy the effects of dnPI3K on Chordin-mediated neural induction. Image published in: Geary L and LaBonne C (2018) © 2018, Geary et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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