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Figure 7—figure supplement 2. MAPK activation is required for neural crest factor expression at mid-neurula stages.(A–D). In situ hybridization examining FoxD3, Sox9, and Snail2 expression in mid-neurula stage (stage 15) embryos injected with dnRaf (A) or treated with the PI3Ki (Wortmannin) (C). Western Blot analyses were conducted with stage nine animal cap explants dissected from dnRaf injected sibling embryos (B) or with mid-neurula stage (stage 15) whole embryos treated with PI3Ki (D). Asterisk denotes injected side, marked by staining of the lineage tracer β-galactosidase (red). Blocking MAPK activation causes a loss of FoxD3, Sox9, and Snail2 expression. Image published in: Geary L and LaBonne C (2018) © 2018, Geary et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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