Xenbase Image ID: 171474

Additional file 1: Figure S1. Validation of tissue induction (A) PCR on RNA extracted from stage 15 animal cap tissue induced to become either neural or NC showed that tissue was induced efficiently. The NC marker Snail2 was only expressed in the NC animal cap tissue whilst the neural marker Sox2 was enriched in the neural tissue and epidermal keratin was enriched in the ectoderm (Ecto) sample. Histone H4 was used as a positive control and Bracyury for a control of mesoderm contamination Whole embryos (WE) were used a positive controls for all genes. (B) WISH for the NC marker Sox10 on stage 15 induced animal caps. This is further confirmation of induction of NC tissue as expression is only evident in NC animal caps. Image published in: Ward NJ et al. (2018) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page