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Figure 3. Ca2+ transients and their localization in DMZ explants. (a) Preparation of DMZ explants including the LEM. (b) Snapshots of time-lapse imaging of the Ca2+ dynamics in DMZ explants. The FRET ratio of yellow cameleon-nano was converted to pseudocolours (bar at right). White arrowheads indicate calcium transients. Scale bar: 100 μm. (c) Frequency of calcium transients during LEM migration in DMZ explants. n = 22 embryos Error bars indicate s.e. ± Student’s t-test, **P < 0.005. Image published in: Hayashi K et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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