XB-IMG-171504
Xenbase Image ID: 171504
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Figure 4. Ca2+ transients are required for LEM migration. (a) Ca2+ transients in DMZ explants treated with BAPTA-AM for 3 hours. Red bars indicate average values. DMSO: n = 7 embryos and BAPTA-AM (50 μM): n = 8 embryos. Mann–Whitney U-test, **P < 0.005. (b) Location of calcium transients during LEM migration in BAPTA-AM- or DMSO-treated DMZ explants. n = 8 embryos. Red bars indicate average values. Mann–Whitney U-test, **P < 0.005, *P < 0.05, n.s.: No significance. (c) Migration of DMZ explants treated with or [without] DMSO. Migration was suppressed by BAPTA-AM treatment. Scale bar: 100 μm. (d) Relative migration distance in DMSO- or BAPTA-AM-treated DMZ explants. Values were normalized to the migration distance of DMSO-treated explants. Error bars indicate s.e. ± Student’s t-test, **P < 0.005. (e) Migration velocity of leader cells in DMSO- or BAPTA-AM-treated DMZ explants. Error bars indicate s.e. ± Student’s t-test, **P < 0.005. (f) Migration velocity of follower cells (≥4th) in DMSO- or BAPTA-AM-treated DMZ explants. Error bars indicate s.e. ± Student’s t-test, n.s.: No significance. (g) Left: Snapshot from time-lapse imaging. Cytochalasin-D (1 μM) treatment suppressed the migration activity in DMZ explants. Right: Ca2+ imaging of DMZ explants treated with Cytochalasin-D (1 μM). White arrowheads indicate Ca2+ transients. (h) Location of Ca2+ transients during 3 hours of Cytochalasin-D (1 μM) treatment. Error bars indicate s.e. ± Student’s t-test, **P < 0.005. Image published in: Hayashi K et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |
