Xenbase Image ID: 171507

Figure 1. a, Schematic of X. laevis and X. tropicalis cross-fertilization outcomes. b, Developmental timing in X. tropicalis and te × ls hybrid embryos. Average is plotted for each time point. Error bars, s.d. c, Representative images of X. tropicalis and te × ls hybrid embryos at stages 3 and 10 from experiments in b (n = 16 X. tropicalis and n = 16 te × ls hybrid embryos from four independent experiments). Arrow indicates vegetal cells where death initiates. d, Schematic of animal cap assay and images of at 2, 9, and 16 h after isolation. Six animal caps were imaged and identical results were obtained in three different experiments. Scale bars in c and d, 200 μm. e, Images showing haploid phenotype following fertilization of X. tropicalis eggs with ultraviolet-irradiated sperm. Identical results were observed in n = 3 experiments. f, Time-lapse images of dividing cell in a te × ls hybrid animal cap (Supplementary Video 5). Arrow indicates a mis-segregated chromosome. Mis-segregated chromosomes were observed in n = 3 live te × ls hybrid animal caps in three experiments. Time is in minutes:seconds. g, Immunofluorescence images showing chromosome bridges, mis-segregated chromosomes, and micronuclei throughout te × ls hybrid embryos. Scale bars in f and g, 10 μm. Quantification of n = 81 X. tropicalis and n = 78 te × ls hybrid anaphases in n = 17 and 16 embryos, respectively, from four datasets obtained from three experiments presented as averages ± 1 s.d., show a significant difference by Fisher’s 2 × 3 contingency test (P = 0). Quantification of micronuclei in te × ls hybrid embryos is detailed in Extended Data Fig. 1b. Image published in: Gibeaux R et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Larger Image Printer Friendly View

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