Xenbase Image ID: 171508

Figure 2. a, Fluorescence images of spindles formed around X. tropicalis, le × ts hybrid, and X. laevis chromosomes in X. tropicalis egg extract. Scale bar, 10 μm. Quantification for n = 147, 103, and 156 spindles quantified for X. tropicalis, le × ts hybrids, and X. laevis embryo nuclei, respectively, from three different egg extracts, is presented in Extended Data Fig. 1e. b, Fluorescence images of X. laevis chromosomes stained for CENP-A or Ndc80 following replication in X. laevis or X. tropicalis egg extract. CENP-A and Ndc80 labelling was quantified from six experiments (three biological replicates in two technical replicates), a total of n = 1,792 and n = 1,959 chromosomes, respectively, in X. laevis extract, and n = 2,692 and n = 1,930, respectively, in X. tropicalis extract. Scale bars, 5 μm. Box plots show the six experiment percentages as individual data points, their average as thick lines, and 1 s.d. as grey boxes. Ninety-five per cent confidence intervals are 96.2 ± 1.9% in X. laevis extract compared with 82.7 ± 5.7% in X. tropicalis extract for CENP-A, and 83.5 ± 6.1% compared with 71.1 ± 6.0% for Ndc80. P values were determined by two-tailed heteroscedastic t-test. c, Circle plot of whole-genome sequencing data for te × ls hybrid embryos aligned and normalized to the genomes of X. tropicalis (blue) and X. laevis (green), with underrepresented genome regions in black. d, Expanded view of chromosome (Chr.) 3L and 4L breakpoints with deleted regions (Del.) indicated in two biological replicates (Rep.). Image published in: Gibeaux R et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Larger Image Printer Friendly View

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