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Supplementary Figure 1 CPLANE pulldowns. (a) Tandem affinity purification of Inturned interacting proteins. Lysates from murine IMCD3 collecting duct cells stably expressing LAP-Intu were subjected to tandem affinity purification and silver stain to visualize major interacting species. Molecular mass markers are indicated on the left. (b) Tandem affinity purification of interacting proteins for Inturned, Fuz and Wdpcp. “s” indicates the tagged proteins. Molecular mass markers are indicated on the left. The calculated molecular masses for these proteins are: mIntu, 104.8 kDa; mFuz, 45.5 kDa; mWdpcp, 81.7 kDa. (c-e) Tables showing an extracted subset of data from Supplementary Data Set 1 and highlighting key findings reported here. Numbers indicate peptide spectral matches for preys retrieved after pulldowns with indicated baits. The “Other Ciliopathy” column reflects combined data from pulldowns of Ahi1, Cep290, Invs, Iqcb1 and Nphp4. Image published in: Toriyama M et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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