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Figure 3. Validation of B55α and B55β-associated proteins. B55α or B55β pull-down was performed in Xenopus egg extracts. A control pull-down was performed using the same volume of amylose beads that were not conjugated with proteins. The extract input (approximately 20%), control (ctr) pull-down and B55α or B55β pull-down samples were analyzed by immunoblotting using PP2A, Mcm2, Ube3a, Mastl, Plk1, Incenp, Smc2, and MBP antibodies. Image published in: Wang F et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |